Journal: bioRxiv

Article Title: Systems-level phosphoproteomics reveals conserved and subunit-specific STRIPAK signaling networks in Cryptococcus neoformans

doi: 10.64898/2026.01.05.697761

Figure Lengend Snippet: STRIPAK complex far9 Δ and far11 Δ mutants exhibit significant defects in growth, stress response, and sexual development. (A) WT (H99α) and isogenic far9 Δ and far11 Δ strains, along with their respective genetically complemented strains, were serially diluted and plated on YPD at 25°C, 30°C, and 37°C; RPMI; and YPD supplemented with 1 μg/mL FK506/100 μg/mL cyclosporine A (CsA), 1 M sorbitol, 1.5 M potassium chloride (KCl), 1.5 M sodium chloride (NaCl), 0.01% SDS, 1.5 mg/mL calcofluor white (CFW), 0.5 mg/mL caffeine, or 1% Congo red—each at 30°C. Images were taken after 2 days of incubation. (B) Fluconazole Etest to analyze drug susceptibility in WT (H99α), WT/WT (KN99α/ a ), far9 Δ, and far11 Δ strains. Cells were grown in an overnight culture in YPD to saturation, then spread onto YPD plates before adding FLC Etest strips. Plates were incubated at 30°C and images were taken after 48 hours. (C) WT (H99α), far9 Δ, and far11 Δ were serially diluted and plated onto Niger seed or L-DOPA agar medium to induce melanin production. The lac1 Δ mutant was included as a negative control. Plates were incubated at 30°C for 5 days. (D) Reduced mating efficiency of far9 Δ and far11 Δ. Cells were co-cultured with wild-type cells of the opposite mating type (KN99 a ) on MS plates. An H99α x KN99 a cross served as a control. Images of mating patches and basidia were taken after 5 weeks’ incubation at room temperature.

Article Snippet: Fluconazole Etests were performed using 0.016–256 μg/mL MIC test strips (Liofilchem 921470).

Techniques: Incubation, Mutagenesis, Negative Control, Cell Culture, Control